UV Light Path

The UV light path is best explained with a diagram.

• The excitation light is projected onto the protein crystal via the condenser lens – dichroic – UV objective optical chain. (Blue arrow)
• The tryptophan amino acid flouresces under the UV illumination. (Red arrow)
• The emission light passes through the UV objective, dichroic, emission filter, and is focused on the CCD sensor by a UV relay lens.

Key: Blue lines indicate excitation light, red lines indicate emission light.

The Diagram of How UV Imaging Works

Related Topics

• UV Imaging (Optional)
• UV Absorption Imaging
• UV Compatible Plates