UV Light Path
The UV light path is best explained with a diagram.
- The excitation light is projected onto the protein crystal via the condenser lens - dichroic - UV objective optical chain. (Blue arrow)
- The tryptophan amino acid flouresces under the UV illumination. (Red arrow)
- The emission light passes through the UV objective, dichroic, emission filter, and is focused on the CCD sensor by a UV relay lens.
Key: Blue lines indicate excitation light, red lines indicate emission light.
The Diagram of How UV Imaging Works