Concepts
Profile
A profile is a set of experiment settings that can be used for concentrating a sample or performing buffer exchange. You can set up a profile from the AUTO PROFILE menu on the home screen. Learn more about Setting Up Auto Profile.
Prime
The priming process is the first step of concentrating your sample solution. The system will fill the filter chip with buffer to remove air bubbles formed during the concentration process.
Concentrate
The concentrating process will be performed after the priming process is done. The sample will be filtered using tangential flow filtration across the membrane in the filter chip.
Buffer Exchange
This is an optional process if you want to perform buffer exchange or desalting with concentration. The reagent type can be the same or different as the carrier reagent.
Liquid Compatibility
You can use the following reagents to clean µPULSE instrument, in order of preference:
- 0.1 M of NaOH (sodium hydroxide)
- 6 M Guanidinium chloride
- 8 M Urea
Important: All cleaning solutions must be filtered through a 0.22 μm pore filter or similar.
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