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FLO i8 2.7

  • Specifications
  • Instument and Software Overview
    • Instrument Overview
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  • Using the FLO i8 System
    • Concepts
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      • Pipetting Parameters
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      • Getting Started with FLO i8
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      • Creating a New Protocol
        • Creating a New Protocol
        • Creating a Simple Transfer
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      • Executing a Protocol
        • Executing a Protocol
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  • FLO i8 2.7
  • Using the FLO i8 System
  • Concepts
  • Pipetting Parameters

Pipetting Parameters

You can easily modify the pipetting settings through the Pipetting Parameters feature that can be accessed and managed through the Pipetting Profile menu. By default, the FLO i8 provides you with the Factory Profile and Factory SD (Serial Dilution) Profile in the Pipetting Profiles Explorer page, that you can use in your experiment.

Important: Both factory profiles in the Pipetting Profiles Explorer page can be used in the protocols, but cannot be modified, removed, or copied to other users.

Pipetting Profiles Explorer Page

Pipetting Profiles Explorer Page

 

There are 2 pipetting profile categories:

  • Regular Pipetting Profile — The regular pipetting profile can be used with the Simple Transfer pipetting steps. There are four tabs in the Regular Pipetting Parameters settings: Tips, Mixing, Optimization, and Advanced.

Regular Pipetting Parameters Settings

Regular Pipetting Parameters Settings

  • Serial Dilution Pipetting Profile — For Serial Dilution pipetting steps, use the Serial Dilution Pipetting Profile settings. There are three tabs in the Serial Dilution Pipetting Parameters settings: Serial Dilution Settings, Sample Pipetting Parameters, and Diluent Pipetting Parameters.

Serial Dilution Pipetting Parameters Settings

Serial Dilution Pipetting Parameters Settings

 

Each tab in the Pipetting Parameters Settings includes predefined Aspirate and Dispense settings, which determine the pipetting settings during both operations. For more information, see Using the Pipetting Parameters.

To access the pipetting parameters:

Choose from the following options to access the pipetting parameters.

From the Pipetting Profiles Explorer page Expand
  1. Click the Application button, select the Explore menu, and then click Pipetting Profile.
  2. In the Pipetting Profiles Explorer page, select a pipetting profile either from the My Regular Pipetting Profile or My Serial Dilution Pipetting Profile section, and click OPEN. For quicker access, double click on the selected profile.

Accessing the Pipetting Parameters in the Pipetting Profiles Explorer

Accessing the Pipetting Parameters in the Pipetting Profiles Explorer

 

From the Pipetting Profiles Explorer page, you can add a new profile, modify and manage existing profiles. For more information, see Using the Pipetting Parameters.

Within a pipetting step Expand

While authoring or editing a pipetting step in a protocol, open the pipetting parameters from the Pipetting Parameters tab. See the images below to open the tab from the Simple Transfer and Serial Dilution steps.

Pipetting Parameters Tab in the Simple Transfer Window

Pipetting Parameters Tab in the Simple Transfer Window

Pipetting Parameters Tab in the Serial Dilution Window

Pipetting Parameters Tab in the Serial Dilution Window

Related Topics

  • Using the Pipetting Parameters
  • Creating a Simple Transfer
  • Creating a Serial Dilution
  • Executing a Protocol
FLO i8 2.7
Updated on June 3, 2024

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