How to create new experiments

  1. In Cellmatic Suite, go to Cell Experiments > Create Experiment, and select one of the options below:
    • Custom: Designs an experiment from scratch.
    • Stock: Maintains a cell line continuously using a template.
    • Order: Generates a cell plate using a template.

      Create Experiment

      Create Experiment

  2. Fill out the General Information column on the left column.

    1. Enter a descriptive name for your experiment.
    2. Select the source plate from the Inventory Library—single or multiple plates. Make sure to select the same type of labware.

      Notes:

      • Selecting a plate already assigned to an experiment will stop its current task and be assigned to this new experiment.
      • Selecting multiple plates as the sources of your experiment assigns the same stages and parameters to them at once.
    3. Select the experiment method.
      • Create New Method: Designs a new experiment method from scratch, including defining the task profiles, parameters, and conditions for each stage.
      • Select From Library: Adopts an existing method from the library and applies it to this new experiment. The task profiles, parameters, and conditions of each stage are adjustable according to the experiment requirements.

      General Information

      Fill Out the General Information

  3. Add a task(s) to the stage. Make sure to complete the stage before adding a new one.

    Notes:

    • Media Exchange and Imaging tasks can be combined in one stage.
    • Users can add multiple schedules within a single Media Exchange, Imaging, or Dispense task.
    • Schedules within a task run sequentially, meaning each schedule must be completed before the next one begins. Therefore, the earlier schedule must not have unlimited repetitions.
    1. Select a task at the bottom to add to the stage.
      • Media Exchange, Imaging, and Dispense: Define the execution time when adding these tasks to the stage. 
    2. Select the task profile in the pop-up window and select OK. For the Media Exchange, Imaging, and Dispense tasks, select +Add Schedule to open the task profile window. Make sure to read the description before selecting one. 
    3. Fill out the parameters for the automation to run correctly. 
    4. After completing the parameters, select the Save button. You can still adjust the task profile or parameter by selecting Edit.

      Add Tasks to the Stage

      Add Tasks to the Stages

       

  4. Select a condition for each stage in the Proceed to next stage when dropdown menu, located on the bottom right, before adding a new stage. You can select one of these five conditions to go to the next stage:
    1. All tasks are completed: The experiment moves to the next stage once the current stage is complete. To use this option, the task must have a limited repetition. 
    2. Confluency reaches threshold: The experiment continues to the next stage after achieving the confluency threshold. Do not select this option if the stage has no imaging confluency task.
    3. Time interval is reached: The duration that the experiment should be fulfilled before moving to the next stage. 
    4. User gives action: The active experiment requires the user to select Continue Stage to proceed to the next stage. Refer to the Go to Next stage section for the details.
    5. Specific date & time are reached: The experiment continues to the next stage after the specified date and time. 
    6. All plates are finished: This option is only available for Pooling, indicating the system has finished collecting cells from all source plates.

      Select the Condition to Continue to the Next Stage

      Select the Condition to Continue to the Next Stage

    Notes: 

    • The preceding conditions may appear differently depending on the task.
    • Some conditions like Confluency reaches threshold, Time interval is reached, and Specific date & time are reached require unlimited repetitions.
  5. Repeat steps 3-4 to add new stages and tasks to your experiment.
  6. Select one of the following options to store your experiment:
    • Save: Stores your experiment.
    • Save to group: Stores your experiment in a particular group. The group must be created before setting up the experiment. 
    • Save & add new method: Stores and makes your experiment as a template.
Note: Tasks in different stages are executed sequentially, meaning the system completes tasks in one stage before moving on to the next.
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