Setting Up Auto Profile
The µPULSE allows you to create a profile consisting of desired experiment settings for concentrating sample or performing buffer exchange. The system tracks volume as it runs sequences based on the values you have entered when designing a profile.
To set up a new profile, tap AUTO PROFILE on the Home Screen. Then choose between these 3 options:
- Tap the CONCENTRATE tab if you want to concentrate a sample 50 mL or less.
- Tap the CONCENTRATE > 50 ML tab if you want to concentrate more than 50 mL of sample.
- Tap the BUFFER EXCHANGE tab if you want to exchange buffer solution for another from the buffer (right) tube. The reagent type can be the same or different as the carrier reagent.
AUTO PROFILE Menu
Tip: Tap the Help button
to get acquainted with the software and learn about what you can do in the AUTO PROFILE menu.
Creating a New Profile
- Tap AUTO PROFILE on the Home Screen and select one of the options:
To create and run a concentrate profile, follow the instructions below:
- Click the ADVANCED SETUP EXPLORER to change the pressure settings.
- Click EDIT to change settings.
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- In the General Setup tab, the Safety Threshold can be adjusted. You can also choose the priming option between BUFFER or SAMPLE and define the Priming Volume.
- Click the CYCLE SETUP tab to to adjust the diaphragm pressure, return valve pressure, and fill/empty time.
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Note: Tap the Edit 
button to adjust Return Valve Pressure.
- Tap SAVE SETUP AS to save the customized settings.
- Click LOAD SETUP to use the saved settings.